CYTIVA 28-9909-44

Superdex 200 Increase size exclusion chromatography columns are well suited for high-resolution analysis and small-scale purification of monoclonal antibodies (mAb) and other biomolecules with Mr~ 10 000 to ~ 600 000.

Enhanced performance: improved resolution and runtime compared to predecessor Superdex 200.

Very high resolution: small bead size and narrow particle size distribution provide high resolution, for high protein purity.

High flow rates: rigid beads give excellent pressure/flow properties.

“Increase”: Cytiva's size exclusion chromatography columns

Superdex 200 Increase is a next-generation, dextran-agarose composite matrix for SEC. With smaller and more rigid beads than their predecessors, Superdex Increase columns deliver higher resolution purification in shorter run times.

A range of pre-packed columns is available to suit a variety of preparative and analytical applications (Table 1-1).

Selectivity for small-scale purification and analysis of monoclonal antibodies

Superdex 200 Increase has a selectivity curve optimized for excellent resolution of antibodies and macromolecules within the range Mr ~ 100 000 to ~ 300 000. It is well suited for detection and separation of antibody monomers, dimers, and aggregates present in monoclonal antibody preparations.

The outstanding resolving power of this resin facilitates protein characterization, including analysis of antibody size variants, assessment of membrane protein size homogeneity, and the study of protein-protein interactions.

Superdex 200 and Superose 6 analysis ranges are complementary

Superdex 200 Increase is optimized to give high resolution separation for proteins with molecular weights in the range Mr ~ 100 000 to ~ 300 000. Superose 6 Increase provides a complementary fractionation range, resolving very large protein complexes and macromolecules that Superdex 200 Increase cannot separate.

Cleaning-in-place (CIP) capabilities

This agarose-based resin is alkali-resistant and supports cleaning-in-place (CIP) procedures. This capability allows the same column to be used for different proteins, with minimal risk of carry-over between samples.

Regular cleaning can also increase column lifetime.

Choose from 3 column sizes

Superdex 200 Increase columns are available pre-packed in three different sizes, to suit varying application needs (Table 1-1).

Column Recommended sample volume Recommended operating flow Efficiency (N/m) Applications

Superdex 200 Increase 3.2/300 4 to 50 µL 0.075 mL/min >48,000 High-resolution analysis and purification of small sample volumes, especially when sample amount is limited and low buffer consumption is important.

Superdex 200 Increase 10/300 GL 25 to 500 µL 0.75 mL/min > 48,000 Standard for high-resolution preparative purification and analysis of small volumes of proteins, especially monoclonal antibodies

Superdex 200 Increase 5/150 GL 4 to 50 µL 0.45 mL/min > 42,000 Rapid purity check, homogeneity analysis and screening of proteins, especially monoclonal antibodies.

Successful use in published research

In addition to their use for antibody purification, Superdex 200 Increase pre-packed columns are also used in a variety of other applications, including:

structural analysis of Salmonella ATPase ligand-bound states by SEC-MAL

analysis of protein oligomerization states

study of replicative helicase machinery

examination of the structure, assembly, and function of cytoplasmic pore filaments

initiation of pulsed gene expression by LytTR transcription regulators