CYTIVA 17-0042-01

Sephadex® G-50 Superfine is a well established gel filtration resin for desalting and buffer exchange of biomolecules >30 000 molecular weight. The Superfine's small bead size give higher efficiency.

• Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
• Suitable for purification of DNA from small molecules by gel filtration
• Small bead size for shorter diffusion distances
• Smallest bead size in the Sephadex® G-50 range

Sephadex® is a gel filtration resin prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex® G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex® G-50 is available in 4 different particle sizes (Coarse, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Coarse and Medium are are preferred for large scale group separations where high flow rates and low operating pressures are required.

• Matrix Cross-linked dextran 
• pH stability, operational1 2–10 
• Flow rate2 < Darcy´s Law 
• Pressure/Flow Specification min 60 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, column 5 cm i.d. 
• Fractionation range (Mr) (globular proteins) 1 × 10³–3 × 104 
• Fractionation (Mp) Dextrans 1 x 10²-5 x10³ 
• Exclusion Limit > 3 x 104 
• Storage 4 to 30°C 
• Autoclavable In wet form (pH 7.0) at 120°C for 30 min