CYTIVA RPN1236

This highly specific antibody to GST is conjugated to horseradish peroxidase and optimized for use in Western blotting with ECL Detection Reagents. The conjugate recognizes multiple epitopes of GST and therefore is not reliant on functional GST for fusion protein detection. It has also been cross-absorbed to E. coli proteins so that GST-fusion proteins can be easily and specifically identified against an E. coli background.

GST is a commonly used expression marker in cloning that is also used for purification and detection of fusion proteins. Detection of GST is simplified because no secondary antibody is required

• Offers the advantages of speed, sensitivity, increased safety, and convenience when used with ECL detection systems. Application tested with ECL and ECL Plus reagents in western blotting for sensitive hard copy results
• Binds with high affinity to GST fusion proteins.
• Also suitable for colorimetric detection with diaminobenzidine (DAB) and tetramethylbenzidine (TMB).
• ECL optimized protocols included.

Application: Offers speed, sensitivity and increased safety for detection of GST tagged proteins 

Estimated shelf life from manufacture date: 3 months 

Solubility: Soluble in cold water and hot water 

Storage conditions: -15°C – -30°C