SIGMA 11635379001
Rapid DNA Ligation Kit
Kit Includes:
1 kit containing 3 components sufficient for 40 reactions (DNA ligation)
For Use With:
Electroporation can be performed after ligation in combination with the High Pure® PCR Product Purification Kit.
Downstream Application:
Covalently joins either sticky-ended or blunt-ended DNA fragments
Millipore Sigma® Rapid DNA Ligation Kit
The Rapid DNA Ligation Kit contains all reagents necessary for ligation. No additional reagents or additives are required. The kit can rapidly ligate DNA with either blunt or sticky ends at +15 to +25°C. Depending on the DNA concentration in the reaction, the ligation products will be either circular (if the DNA concentration is low) or concatemeric (if the DNA concentration is high). Ligated DNA is suitable for direct use in transformation experiments.
- Sufficient for 40reactions (DNA ligation)
- Incubation time: 5 minutes
Features and Benefits
- Fast: ligation takes only 5 minutes.
- Convenient: ligation is performed at +15°C to +25°C.
- Easy: kit contains all buffers and enzymes needed.
- Flexible: kit can be used for all common ligation reactions.
- Reliable: simple procedure and ready-to-use reagents ensure accuracy and reproducibility.
Kit contains:
- Dilution Buffer 5x concentrated
- T4 DNA Ligation Buffer 2x concentrated
- T4 DNA Ligase 5 U/µl
Kit Includes:
1 kit containing 3 components sufficient for 40 reactions (DNA ligation)
For Use With:
Electroporation can be performed after ligation in combination with the High Pure® PCR Product Purification Kit.
Downstream Application:
Covalently joins either sticky-ended or blunt-ended DNA fragments
