RBIOPH R7001

RIDASCREEN® Gliadin is a R5 sandwich enzyme immunoassay based on specific monoclonal antibody to celiac toxic amino acid prolamin sequences to determine gliadin as a measure of gluten in food.

It is applicable for the quantitative measurement of intact gliadin as a measure of gluten in unprocessed and processed matrices from important gluten-free food categories, including rice- and corn-based products, soy, starches, pseudo cereals, legumes, spices, juice, nut nougat crème, cream cheese, pesto, meat, vegetarian meat alternative, cookies, dessert, cake, fish, bread, candies, and potatoes. The sandwich ELISA quantifies intact gliadin from wheat and related proteins from rye and barley. This method is not accurate for quantification of fermented or hydrolyzed gluten.

• Sample Preparation: Homogenization and extraction
• Incubation time: 1hr 30min at Room temperature
• Allergen swabbing using RIDASCREEN® Allergen ELISAs.
• RIDASCREEN Gliadin in combination with RIDA® Extraction Solution (colorless) (R7098) – only after validation.
• Sample preparation for raw materials with ethanol.
• Sample preparation for polyphenol containing raw materials (e.g. chocolate, cofee, cacao, buckwheat) with fish gelatine and ethanol.
• Validated Matrices: Rice- and corn-based products, soy, starches, pseudo cereals, legumes, spices, juice, nut nougat crème, cream cheese, pesto, meat, vegetarian meat alternative, cookies, dessert, cake, fish, bread, candies, and potatoes
• Detected Analyte: QQPFP epitope that occurs in α-/β-, γ-, and ω-gliadins; B-, C-, and γ-hordeins; as well as γ- and ω-secalins from wheat, rye, and barley