ALPHABIO L12-106-500

Lauryl Sulfate Broth, otherwise known as Lauryl Tryptose Broth, is a liquid medium used for the detection of coliforms in water and wastewater. Coliforms are often used as indicators of fecal contamination in water and bacterial contamination in food products. Enzymatic Digest of Casein offers a source of nitrogen, vitamins, and minerals. Lactose is added to detect its fermentation by coliforms, while the Sodium Lauryl Sulfate acts as a selective agent against non-coliform organisms. Sodium Chloride is used to maintain the osmotic balance of the medium and the Potassium Phosphates act as buffering agents.

1. The powder is homogeneous, free flowing and light beige.

2. Visually the prepared medium is yellow to gold and clear to trace hazy.

3. Expected cultural response after 18 to 48 hours at 35°C.

Formula per Liter:

• Enzymatic Digest of Casein..................20.0g
• Sodium Chloride .................................... 5.0g
• Lactose ...................................................5.0g
• Potassium Phosphate, Monobasic........2.75g
• Potassium Phosphate, Dibasic .............2.75g
• Sodium Lauryl Sulfate.............................0.1g

Store the sealed bottle containing the dehydrated medium at 2 to 30°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original color.