Cytiva® Superdex™ 30 Increase Tricon™ 10/300 GL Prepacked SEC Column

Superdex™ 30 Increase is part of Cytiva's new generation of agarose-based SEC columns called “Increase”, which offer higher resolution purification in shorter runtimes compared with their predecessors. The fractionation range allows preparative separation of peptides, oligosaccharides and other small biomolecules (Mr ~ 100 to ~ 7000) with sample volumes up to 500 µL. These columns also enable protein characterization, including aggregate analysis, size homogeneity analysis, and interaction studies, where high-quality data can be achieved. This agarose-based resin is alkali resistant and supports cleaning-in-place (CIP) procedures. This capability allows the same column to be used for different biomolecules, with minimal risk of carry-over between samples. 

Superdex™ 30 Increase SEC columns are for high resolution, small-scale purification and analysis of small biomolecules with Mr ~ 100 to ~ 7000, such as peptides

• Improved performance – improved resolution and runtime compared to predecessor Superdex Peptide
• Tolerance to repeated harsh cleaning procedures at high pH ensures long column lifetime and minimizes carryover.
• Very high resolution – small bead size and narrow particle size distribution provide high resolution, for high protein purity.
• High flow rate – due to rigid beads giving excellent pressure/flow properties.
• New generation of size exclusion chromatography columns