Cytiva™ Amersham™ ECL Plex Cy3 and Cy5 Conjugated Antibodies

Amersham ECL Plex Cy3 and Cy5 labeled antibodies are part of an optimized system for quantitative analysis using fluorescent Western blotting.

Fluorescence detection is a direct Western blotting method in which a secondary antibody is conjugated to a fluorophore, avoiding the need for ancillary detection reagents.

Amersham Cy3 and Cy5 secondary antibodies are labeled with near-infrared (NIR) fluorophores that emit light at wavelengths of 570 and 670 nm. By using Amersham Cy3 and Cy5 secondary antibodies together with a pair of appropriate rabbit or mouse primary antibodies, you can perform multiplexed experiments.

Key benefits:

• Broad dynamic range
• High sensitivity
• Ability to detect multiple proteins on same blot (multiplexing)
• Wide pH tolerance (3 to 10), making them compatible with most Western blotting buffers
• High photostability
• Signal longevity > 3 months